Applying Theory to Practice Case Study Example | Topics and Well Written Essays - 750 words

Applying Theory to Practice - Case Study Example The case explains that Bosket’s father was serving jail term and his mother was married to somebody else and did not have much to do with the young boy (Agnew, 2008). One of the factors that I believe are important in understanding Willie Bosket’s delinquent behavior is the motivation to commit crime. It seems like the fact that Bosket committed several crimes and got away with them without serious consequences reinforced his motivation to commit more crimes. The money he got from robbing people acted as motivation for Bosket to commit crimes. The perceived power he got from carrying a gun was another element that added onto Bosket’s motivation to commit crime. The gun made Bosket feel that he would subdue his victims easily and rob them or do whatever else he needed to do to them (Bohm, 2010). Another factor that is important in understanding Bosket’s delinquent behavior is his history of crime. Bosket committed many crimes and confronted law enforcement agencies severally. It is possible that the experience that Bosket got from his history of crimes made him feel that he was becoming a master at what he was doing thus enjoying committing more crimes. Something about Bosket’s history of crime that could explain his delinquency is a likelihood of weakness in the law enforcement system. Weak law enforcement allows juveniles like Bosket to advance their delinquency since they come to believe that nothing serious will happen to them (Agnew, 2008). Absence of parents to Bosket’s from his immediate environment is another important factor in understanding the juvenile’s delinquent behavior. When parents live with their children in their immediate environments they teach their children to conform to norms and values, which discourage crime and this, is one of the things that Bosket was missing. Absence of Bosket’s father must have had an influence in Bosket’s delinquency

Managerial Accounting and Business Analysis Essay

Managerial Accounting and Business Analysis - Essay Example 220.65 Over draft Bank Balance &cash 100 354.01 1701.67 Total current assets 100 133.05 65.96 Total fixed assets 100 377.74 187.27 Total assets 100 56,91 66.17 Account payables 100 117.67 0.32 Retained income 100 216.71 130.16 Total liabilities and equity 100 156.68 188.85 Income Report for Bell way Plc Figures in million of pounds 2003 000 000 2004 2005 Turnover 954,197 1,092,571 1,178,063 Gross Income 739,479 829,598 280,402 Trading income 172,762 213,277 229,448 Interest received 1,427 1,361 2,267 Earnings before interest and tax 169,251 205,530 218,163 Interest paid 5,811 10,477 13,474 Taxation 50,687 61,700 65,400 Earnings after tax 169,251 205,530 152,763 Dividends 24,166 29,864 37,137 Retained income for the year 94,398 113,971 115,620 Balance sheet for Bell way Plc. 2003 2004 2005 Assets 16,200 16,673 16,203 Stock 478,935 587,635 Over draft Bank Balance &cash 3,468 4,926 703,048 Total current assets 482,327 590,246 697,649 Total fixed assets 16,200 16,200 697,649 Total assets 482,327 590,246 681,446 Account payables Retained income 94,398 113,971 115,626 Total liabilities and equity 482,327 590,246 697,649 If the above results are converted into indexed values form the following results can be obtained. 2003 2004 2005 Assets 100 102.47 109.26 Stock 100 119.56 145.27 Account receivables 100 100100 Over draft Bank Balance &cash 100 135.83... Recommendation according to horizontal analysis: Though the investment decision cannot be taken only on comparison it can serve as one of the criteria. In case of two companies the profits and turnover had shown a trend of increasing. The total current assets of the Telford was not showing consistent trend when compared to Bell way Plc. The account payables in the 2006 were less for Telford and thus it maintained a increase trend in profit. But the decrease in account payables may show affect in the coming year and the increase trend may not be this much stronger. In contrast to Telford the Bell way was increasing the current assets along with account payables done and still maintaining the increasing trend of profit. This tells us about the good marketing, payment, asset increasing and profit retaining strategies and according to comparison it is advisable to invest in Bell Way According to vertical analysis the items in the income account were calculated as the percentage of turnover. This tells us about the healthy and unhealthy trends of profit making, the company is experiencing. In vertical analysis it can be observed that the bank balance, current assets were not following a healthy trend in case of Telford Plc. The liquidation of assets was maintained as cash in telford.

Enterprise Applications Research Paper Example | Topics and Well Written Essays - 500 words

Enterprise Applications - Research Paper Example From this paper it is clear that  supply chain management (SCM) information flow management among and between the supply chain stages with the view of maximizing profitability and supply chain effectiveness. In this case, the supply chain includes all the parties that are either indirectly or directly involved in the purchase of raw materials or products. Unlike in the past where organizations were more focused on quality improvements and manufacturing, a shift has been experienced in the current corporate world towards influencing SCM to include suppliers and customers. Thus, organizations are expected to embrace technologies that would have an effective impact on the management of the supply chains.  As the discussion stresses virtually seamless links of information between and within organizations form an essential part of integrated supply chains. The major role of Information Technology (IT) in the supply chain is thus to establish tight process and information linkages or c reate integrations between organizational functions. Technological advances made in the recent past have made integrated SCM possible. SCM is driven by various factors including visibility, consumer behavior, speed, and competition. Visibility refers to having a clear view of all the areas of the supply chain, up and down.  Changes in consumer behavior are also evident in the market environment as consumer demands have increased, and so has the need for organizations to respond to such needs faster and effectively by enhancing the supply chain.

Phrmaceutical Formulation Essay Example | Topics and Well Written Essays - 3000 words

Phrmaceutical Formulation - Essay Example It has a wide range of applications, like, levosalbutamol inhalers have been designed to help in the treatment of asthma. Pulmonary drug delivery can be used to administer a vaccine like in the case of currently inhaled measles vaccine given by nebilizer. (Molema, 2001 pg 16) Also it can be used to give medication to infants and young children who have a small tidal volume and low inspiratory flow rates by the use of a baby mask which normally is attached to spacer. (Perry, 2012 pg 25)It has been used to treat lung cancer by the means of inhaled chemotherapy which has proven to be a logical approach and also it is used on patients who are very ill who cannot withstand any more pain by inhaling opioids such as morphine and fentanyl. (Sigmund, 2009 pg10) Drugs used in pulmonary drug delivery have multi-component powders with mucoactive compounds and anti-asthmatic medicines bound together in a single particle because of the mucus layer covering the airways of the tracheobronchial struc ture. (Friebel, 2010 pg 20)The substances used in the inhalers must dissolve 100% so as not to block the air gaps to avoid suffocation. (Le, 2001 pg 37) Been affected by the rapid change of technology due to great inventions pharmaceutical industries had to do some research to come up with more efficient drug delivery system like drug powder inhalers. (Zeng, 2001 pg 8) Although drug powder inhalers has greatly boosted the pharmaceutical companies there are challenges that are experienced during its formulation like the cohesive ,adhesive property of drug and carrier aggregation, particle size , morphology of the drug and carrier aerosolization performance and powder formulation properties. (Koning, 2001 pg 40)One of the challenges in pharmaceutical industries is to characterize their various physical behaviors; here the concern is mainly focused on the study of the relations relating the properties of the grain to those of the powder bed. (Wang, 2012 pg 44)The goal is thus to be abl e to control, analyze and improve the manufacturing processes of the powder. (Talton, 1999 pg 60) To improve the aerosolization performance of drug powder inhalers, varieties of carrier and carrier types have to be sampled and studied so as to come up with the best, and the drug ratios are prepared by spray-drying so as to improve the efficiency of the drying procedure and the characterized at last. Again to improve aerosolization two critical physical properties which are the particle size and flowability has to be keenly observed where the particle size should be around 5-6   Ã‚ µm and the angle of repose to be within the ranges of 43 °Ã¢â‚¬â€œ52 ° for the flowability to boost the aerosolization. If this is done well the aerosolization performances can be improve greatly. Cohesion forces of particles used in dry powder formulation is a great nuisance to inhalers because it reduces powder flow and hinders accurate metering hence needs to reduce it and this can be achieved by blending the active pharmaceutical ingredient with a larger inert carrier so as to reduce the high cohesion forces that are normally between the micron sized active pharmaceutical ingredient particles. Adhesion forces are also of great concern in dry powder inhalers because if not catered for they make the patient to find it difficult to deliver the drug from the inhaler to his or her system hence it must be overcome or reduced by great magnitude so as to allow liberation of drug from the device with minimal energy from the patient. The particle size is a challenge in drug powder inhalation

Promotion of Liberal values by states Essay Example | Topics and Well Written Essays - 1500 words

Promotion of Liberal values by states - Essay Example Further it also presents the idea that the spread of liberalism will not necessarily result in shape of complete peace and prosperity in the world. In order to understand the prescribed role of the liberal states by the Liberalism theory it is important to understand the theory. "Liberalism is an ideology, philosophy, and political tradition that holds liberty as the primary political value. Broadly speaking, liberalism seeks a society characterized by freedom of thought for individuals, limitations on power, especially of government and religion, the rule of law, the free exchange of ideas, a market economy that supports relatively free private enterprise, and a transparent system of government in which the rights of minorities are guaranteed. In modern society, liberals favor a liberal democracy with open and fair elections, where all citizens have equal rights by law and an equal opportunity to succeed." (Wikipedia, 2006) The essential characteristic of the liberal theory of the state is the doctrine of jurisdiction. The liberalism theory emphasizes on the limited area of power and authority for the state. Liberalism provides specified boundaries to the state beyond which the state cannot trespass. The principle of the limited power of state is the characteristic, which signifies the theory of Liberalism from Socialism in which the power of state is unlimited and Anarchists, according to whom the state does not have any significance. According to the neo socialist theory the most important factor is the economic efficiency rather than that of individual liberty and limited government. In order to undertake a discussion of the extent to which the use of power in the spreading the Liberal values is justified we will analyze the principles of Liberalism. The first and the foremost important principle of liberal theory are that the state is not superior to all the institutions. In fact state is also a social institution as other social institutions. All the institutions related to the specified field have relatively more power concerning the field as compare to other institutions. State has its own limitations and boundaries. It has to act in those boundaries. (Cooray) War has remained an issue of concern for most of the countries and nations. The formation of the international institutions such as League of Nations and the UN is the part of efforts of the nations towards establishing peace in the world. But still the notion of war is present in shape of the process of fighting terrorism and intervention to establish democracies. In case of the attack of U.S and its allies at Iraq and the refusal of the United States to follow the suggestion of United Nations to resolve the matter through peaceful talks, denies the first role of the superiority of the institutions on the state. United States used her economic dominance and military superiority in the world to make all the countries, including its enemies and allies to support the decision of war against Iraq through using force. "The second principle of the liberal theory of the state is that the state ought to respect the fault principle." (Cooray) According to the fault principle the state cannot punish an individual or cause him any harm with

Core Competency Essay Example for Free

Core Competency Essay Walt Disney once noted: â€Å"I only hope that we don’t lose sight of one thing – that it was all started by a mouse†. (Disney Dreamer, 1998). Walt Disney’s original core competence was cartoons and animated movies. By combining Imagineering with engineering Disney’s company reached unparalleled success with the creation of the first full length animated movie. This success led to new ideas and one of them was to open a park, a different kind of park. In Disneyland Walt used new technology to bring his characters to life. He called them â€Å"Animatronics†. (Magical Kingdoms, July 2008) Together with unique storytelling and high quality of service Walt Disney created a magical environment for his guests which none of the competitors could quite duplicate. It became Walt Disney’s core competency. Disney once said: â€Å"Anything that has the Disney name to it is something we feel responsible for.† (Disney Dreamer, 1998) This competency is driven by superior Disney products and most of all by cast members and their renowned guest service. It’s always been a challenge to keep up with high expectations of the guests. To continue to improve its business Disney has set up new cast member standards in the form of the Basics (Meeting the new standards, December 2007). The new standards expect cast members to create and reinforce â€Å"magical† experience for the guests by being approachable, engaging, and willing to go above and beyond. As the company developed, many new lines of businesses have been added such as retail, media, and sports. The danger has been to overextend and lose track of what the company does best. In order to stay competitive the company had to realign and shifted attention from retail to those which are the cornerstone of the company – intellectual property. To reinforce its animation business Disney purchased Pixar in 2006. In the acquisition statement it read: â€Å"Animation is the foundation upon which the Company was built and for years was a core competency. However, in recent years Disney placed less emphasis on animation and, as such; the Company was not at the forefront of the digital revolution and did not produce many animation movie hits. With Pixar, Disney is now further on the technology curve and can  exploit some synergies in marketing, production, and distribution.† (Walt Disney Company release, January 2006). In 2009 Disney purchased another company, Marvel. This move further enhances Disney’s competitiveness and extended the collection of its characters. (CNN, August 2009). The special strength of the company is diversification. In addition to four parks in the US Disney currently has a park in Europe, plus two parks and one more on the way in Asia. Disney’s largest sales revenue is from media sold outside the US. Disney has its own cruise line with regular operation of two boats and is adding two more in January of 2011. (Cruise Talk, June 2010) Many regular Disney guests own part of the Walt Disney World resort through â€Å"Disney Vacation Club†, the company’s timeshare program. Disney’s tour operation business â€Å"Adventures by Disney† offers guests special tours around the world. (Adventures by Disney, n.d.) Everything the company does carries the special â€Å"Disney touch† – a seal of quality and outstanding service. Though the company has some challenges with the economic downturn, it continues to grow leaning heavily on what the company does best providing â€Å"magical† experiences to guests and consumers. References: Disney Dreamer (1998), Walt Disney Quotes. Retrieved from: http://www.disneydreamer.com/walt/quotes.htm Josh Buchanan, Matthew Covarrubias, James Gills, T.J Lovejoy, Craig Wuollet, Meeting the new Standards (December, 2007), Executive Summary, P. 3 Retrieved from: http://www.slideshare.net/jamesrg6/disneypaperfinal Walt Disney Company release (January, 2006) Comments on Acquisition of Pixar Animation Studios. Retrieved from: http://www.dbrs.com/research/206997/walt-disney-company-the/comments-on-acquisition-of-pixar-animation-studios.html David Goldman (August, 2009), CNN, Disney to buy Marvel for $4 billion Retrieved from: http://money.cnn.com/2009/08/31/news/companies/disney_marvel/index.htm The History of Disney’s Animatronics (July, 2008), Disney Blog at Magical Kingdoms Retrieved from: http://www.magicalkingdoms.com/blog/2008/07/08/the-history-of-disneys-audio-animatronics/ Mr. Papa (June, 2010) Cruise Talk Retrieved from: http://cruisetalk.org/2010/06/disney-cruise-line-adds-final-building-block-to-new-cruise-ship-disney-dream.html Adventures by Disney, Retrieved from: http://abd.disney.go.com/abd/en_US/index?name=HomePage

Analysis Of The UV Visible Spectroscopy Biology Essay

Analysis Of The UV Visible Spectroscopy Biology Essay Purpose of this term paper is to describe working principle, instrumentation, data collection and data analysis of the UV-Visible spectroscopy which is also known as electron spectroscopy. In working principle, Beer-lambert law correlate absorption of light to concentration of substance in solution. Parts of UV-Visible spectrometer have been described. Data analysis and data collection section describe how data collected by system and what type of information we can get from the data collected from UV-Visible spectroscopy. In the end we conclude what we learn from the project. Introduction: In spectroscopy, matter is been exposed to radiative energy (light, high energy electron, high energy ions etc.), at that time it can interact with matter according to atoms or molecule are present in system. Depending on its interaction with material gives different information about the matter. In short, spectroscopy is the study of the interaction between matter and radiated energy. Absorption, emission, elastics scattering, inelastic scattering are major interaction between radiative energy and matter. In absorption, radiated energy is absorbed by matter. In emission, electron will excite and goes to higher energy level, then when it goes to ground state it will emits electromagnetic waves. When electron and radiative energy interact, but they dont exchange energy, this type of interaction are called elastic scattering, and if electron and radiative energy exchange energy, then this type of interaction are called nonelastic interaction. The selection of the spectroscopy to analysis the sample is depending on what is present in the sample. For example, if atoms of sample are targeted to analysis, X-ray spectroscopy and XRF (X-ray fluorescent) are useful. If molecules of sample are targeted to analysis, Infrared, Raman, visible, UV-visible spectroscopy, and fluorescence spectroscopy are useful. Raman and Infrared spectroscopy are complementary to each other. Same way, UV-visible spectroscopy is complementary to fluorescence spectroscopy. UV-Visible spectroscopy usually used for quantitative analysis of different molecule like transition metal ions, highly conjugated organic compound, and biological macromolecule. UV-Visible spectroscopy use UV light and visible light for analysis of sample. Range of Visible light is 400nm to 800nm. Where UV light has range from 400nm to 200nm which usually used in spectroscopy. Energy associate with 800 nm visible light is 36 kcal/mole. Energy associated with 400 nm visible light is 72 kcal/mole. UV light used in UV-Visible spectroscopy is up 200 nm because smaller then this range it is difficult to handle. So, UV light which has energy less than 200 nm doesnt use frequently. UV light which has wavelength 200 nm, energy associated with it is 143 kcal/mole. Figure 1: Energy band diagram of molecule here it shows HOMO and LUMO (bonding and anti-bonding) energy level Figure 1 is showing general energy band diagram which has energy level HOMO (highest occupied molecular orbital), LUMO (lowest unoccupied molecular orbital). HOMO is also known as bonding energy level. And LUMO is also known as anti-bonding energy level. In this diagram, shows some exciting possibility in molecular electron from lower energy orbital to higher energy orbital. However, from UV-Visible spectroscopy, electron gain only enough energy to excitation from à Ã¢â€š ¬ (bonding) to à Ã¢â€š ¬* (anti-bonding) energy level or from n (non-bonding) energy to à Ã¢â€š ¬* (non-bonding) energy level. For other excitation, it will require even higher energy that UV or Visible light can provide. So, using UV-Visible spectroscopy we can measure first two left hand sides to excitation from diagram. UV-Visible spectroscopy is also known as electronic spectroscopy because it measure absorption of light by electron. When sample molecules are exposed to light having an energy that equals a possible electronic transition within the molecule from HOMO to LUMO, some of the light energy will be absorbed as the electron is promoted from lower energy à Ã¢â€š ¬ orbital to a higher energy orbital like à Ã¢â€š ¬* orbital. An optical spectrometer records absorption at each wavelength and present as graph of absorbance vs. wavelength. Range of absorbance ranges from 0 (no absorption) to 2 (99% absorption) calculate by spectrometer. Here in UV-Visible spectroscopy, Beer-Lambert law has been used to find concentration of absorbing solute in the solution. When a light passes through a solution, due to interaction with material some of the light might be absorbed and the remaining light transmitted through the solution. The ratio of the initial intensity (entering the sample (Io)) and final intensity (exiting the sample (It)) of light at a certain wavelength is defined as the transmittance (T). Most of the time it has been expresses as percent transmittance. And the absorbance (A) of a sample is the negative logarithm of the transmittance. Equation : Beer-Lambert law which correlate transmittance to initial intensity and final intensity Equation : Beer-Lambert law which correlate Absorption to transmittance Here, Io is initial intensity (entering intensity) and It is final intensity (exiting intensity), T is transmittance, A is absorption. The absorbance of a sample at a given wavelength is equal to the absorptivity of the substance, path length and concentration of the substance. Value of the absorptivity of the substance depends on the wavelength. For different wavelength, value of the absorptivity is different. The path length is the distance the light travels through the sample. Equation : Beer-Lambert law which correlate absorptivity, path length, concentration of substance Here, is absorptivity of the substance, l is path length; and c is concentration of the substance. Commonly, and l are constant for experiment because depending on material is fix value and experiment length of path (l) is also fix for each experiment. So, using these equations we can calculate the concentration of substance in given sample. Instrumentation: Figure 2: Working principal of UV-Visible spectroscopy [3] Ultraviolet (UV) and Vis light spectroscopy has been shown is figure-1. This device contains UV light source and visible light source, slits, Diffraction grating, filters, mirrors, reference cuvette, sample cuvette, lenses and detectors. Light source: This device has two light sources. Depending on the sample either UV or Visible light source will be used. Using Mirrors light will be concentrated on Diffraction gritting. Usually UV light source has range from 200nm to 400nm, and visible has range of 400nm to 800nm. For UV light source, Hg bulb is used. And For Visible light source, Tungsten is uses. Diffraction gritting and filter: Diffraction gritting converts light source into its component wavelength light. Prism can be used instead of diffraction gritting. Then Created single wavelength light is given to half mirror Half mirror: Half mirror is special kind of mirror which can provide two same intensity output from single input sources. In our device, it will be used to provide same intensity single wavelength light to the reference cuvette, and sample cuvette which contain only solvent. Solvent can also interact with the sample. So it is necessary to measure light interaction with solvent which later can be remove from sample which contain solvent and solute to measure light interaction with solute which is area of interest. Reference Cuvette and Sample Cuvette: In UV- Vis spectroscopy, it is very important to compare intensity to get transmission. Light is passes through the both cuvette. Absorption is done at this stage; level of absorption will depend on the sample and the reference themselves. Lens and Detector: Lenses will be used to focus and magnify the output reference beam (I0) and output sample beam (I). Here I0 should be absorbed just little Detector will be used to detect these signals and convert into electrical signals which can be further understand using software and computer. Figure 3:   Shimadzu 1650PC, UV-visible Spectrophotometer. [2] Data Collection [4] [5]: Instrument was SHIMADZU UV 1601. Absorption of liquid and thin film can be measured by this instrument. Liquid Sample For liquid or solution, cuvette is used. It is required reference solution containing cuvette, and sample containing cuvette. In reference cuvette, it will have only solvent. Using this data, absorption for cuvette and solvent can be understood. Using data of sample containing cuvette, absorption for cuvette, the sample and solvent can be understood. Surface of cuvettes must be cleaned after filling the liquid to make sure surface does not have any dust particles. Using software, Method is needed to be defined. In our Method, wavelength range is 300 nm to 1100 nm; scan speed is medium; sampling interval is 1 nm; scan mode is single. A 1st need to do is measure reference (baseline, solvent only). It is also important that reference cuvette is inserted in reference stage not in sample stage. Mounting of cuvette is very important. Cuvette has two types of surface. Cuvette has two transparent sides and two semi-transparent sides. Transparent sides need to be aligned so light beam can enter and exit from transparent sides. Load the reference sample. Define wavelength range (1100-300nm) scan in software. It will take about 2 min to finish scan for reference. It is also important to do it reference scanning process again if you change the type of the sample, cuvette or solvent. Secondly, low concentration seldibrdge (2.9*10^-6 mole/L) chromophore sample will be loaded in another cuvette and it will be loaded in to sample stage. In software, start scanning button is clicked. Scanning of the sample will also take about 2 min. When scanning is finished new window will appeared and will ask for file name and file description. Now on screen you will able to see typical graph absorption spectrum of seldibrdge chromophore. Computer will assign some peaks. To access this information peak button on screen will be pressed. After that computer will provide table which include Wavelength and Absorption. This table and concentration of the sample is useful to calculate extinction coefficient or molar absorptivity. This calculation will be done by Beer-Lambert Law. Third, high concentration seldibrdge chromophore (2.9*10^-5 mol/L) sample will be loaded in another cuvette and it will be loaded in to sample stage. Concentration is almost 10 times more than low concentration. It will be loaded in to sample stage. In software, start scanning button is clicked. Scanning of the sample will also take about 2 min. Data can be stored by using Data Print Table extension and it will information in notepad. Thin film Sample is dissolved in polymer matrix and applied on glass substrate. For this process, glass substrate is used as sample. It is very important to not to touch surface of the glass substrates. It is also required to make sure it does not have any dust particles on it. Then load reference sample in reference stage and press baseline button on computer screen. After that, sample is put on sample stage. It is important to orient the sample surface side, so light can directly interact with thin film first rather than glass substrate. Then press start on computer screen. Data table and Spectrum chart will be provide by computer. Data can be saved as the above description. In thin film, concentration is unknown, so extinction co-efficient cannot be calculated. For thin film maximum absorption is important factor. Data analysis: Chromophore is part of molecule which is responsible for its color. So, during UV-Visible spectroscopy electrons in chromophore are interacting with light. Table 1 is giving data about different chromophore. Chromophore may be present in solvent. So, we require selecting proper solvent to measure chromophore of solute. Not all the solvent can be used in UV-Visible spectroscopy. For example, oxygen non-bonding electrons in alcohols do not give rise to absorption above 160 nm. So, we can use as solvent for UV-Visible spectroscopy. Common solvent can be used in UV-Visible spectroscopy are Hexane (alkane), ethanol (alcohol), water. However, if we used UV light which has wavelength are lower than 200 nm, then we cant use alcohol because it create very sharp peak. Chromophore Example Excitation ÃŽÂ »max, nm Ɇº Solvent C=C Ethene à Ã¢â€š ¬__>  Ã‚   à Ã¢â€š ¬* 171 15,000 Hexane Cà ¢Ã¢â‚¬ °Ã‚ ¡C 1-Hexyne à Ã¢â€š ¬__>  Ã‚   à Ã¢â€š ¬* 180 10,000 Hexane C=O Ethanal n  __>  Ã‚  Ãƒ Ã¢â€š ¬* à Ã¢â€š ¬__>  Ã‚  Ãƒ Ã¢â€š ¬* 290 180 15 10,000 hexane hexane N=O Nitromethane n__>  Ã‚  Ãƒ Ã¢â€š ¬* à Ã¢â€š ¬__>  Ã‚  Ãƒ Ã¢â€š ¬* 275 200 17 5,000 ethanol ethanol C-X X=Br          X=I Methyl bromide Methyl Iodide n__>  Ã‚  Ãƒ Ã†â€™* n__>  Ã‚  Ãƒ Ã†â€™* 205 255 200 360 hexane hexane Table 1: Some measured data by UV-Visible spectroscopy. As describe in introduction, using transmittance, we can calculate concentration peak. However, all the data in computer give form of absorbance vs. wavelength or Ɇº vs. wavelength. Sometime value of log Ɇº is taken in place of Ɇº. There are some graph obtain from UV-Visible spectroscopy. Both Ɇº (molar absorptivity) and A (absorption) are changing with different wavelength. Figure-4 is absorbance vs. wavelength graph for C5H6O. Figure 5 is graph of Ɇº vs. wavelength for different conjugate compound which has same chemical formula. In graph, each peak represents a certain excitation. Figure 7 shows two different excitation, first excitation is from à Ã¢â€š ¬ to à Ã¢â€š ¬* and second excitation is from n to à Ã¢â€š ¬*. Both excitations have pick value at different wavelength. For excitation à Ã¢â€š ¬ to à Ã¢â€š ¬*, maximum Ɇº value is around 250 nm. And for excitation n to à Ã¢â€š ¬*, maximum Ɇº value is around 300 nm. From figure 5 and 6 we can say that as number of Chromophore, curve shift to longer wavelength; however number of picks doesnt change if Chromophore is same. However, for aromatics compound, as ring increase, number of pick are increasing. Due to different Chromophore present in molecule, it will effect on maximum absorption wavelength. There different terms are assigned for different type of shift which can see in table 2. Figure 4: UV-Visible spectroscopy results for C = C and C = C Chromophore for particular chemical compound at specific pH, and solvent. [1] Figure 5: UV-Visible spectroscopy results for conjugated compound. [1] Figure 6: UV-Visible spectroscopy results for conjugated compound [1] Figure 7: UV-Visible spectroscopy results for C = C and C = O Chromophore for particular chemical compound at specific pH, and solvent. [1] pH of system is also effect on absorption peak. Diluted Copper sulfate solution is very light blue. However, if you add ammonia which will change pH more than 7, color of solution will change due to change in absorption peak and intensity. Following figure 8, is UV-Visible spectra of phenolphthalein at different pH value. Figure 8: UV-Visible spectra of phenolphthalein (0.103 mmol cm-3) at pH 13 solid light line, pH 9 solid dark line, pH 8 dash light line, pH 4 dashed dark line Nature of Shift Descriptive Term To Longer Wavelength Bathochromic To Shorter Wavelength Hypsochromic To Greater Absorbance Hyperchromic To Lower Absorbance Hypochromic Table 2: Terminology for Absorption Shifts [1] 2 3 1 Figure 8 : Result obtain for Low concentration Liquid seldibrdge chromophore (1), high concentration Liquid seldibrdge chromophore (2), and unknown concentration of seldibrdge chromophore dissolved in polymer matrix and applied on glass substrate (3). [4], [5] Wavelength (in nm) Absorption 780 0.604 510 0.103 475 0.101 342 0.174 1063 .001 540 0.085 492 0.097 389 0.060 For low concentration Liquid seldibrdge chromophore, concentration is 2.9*10^-6 mol/L. peaks and absorption has been shown in following Table. Table 3: Wavelength and absorption for low concentration Liquid seldibrdge chromophore, concentration. [4][5] For higher concentration liquid seldibrdge chromophore, concentration is 2.9*10^-5 mol/L. Absorption of this sample is out of spectroscopy range. So it is required to dilute for further understanding and calculation. For unknown concentration of seldibrdge chromophore dissolved in polymer matrix and applied on glass substrate, concentration is unknown, so further calculation of extinction coefficient cannot be done, but peak absorption can be find. For this sample peak absorption is 0.512 at 808 nm wavelength. Using equation 3, we calculated Ɇº (extinction coefficient or absorptivity) is around 2.1E5 1 / M * cm at maximum absorption. Conclusion: UV-Visible spectroscopy is good for finding concentration or molar absorptivity of of biological macromolecule, organic molecule, transition metal, conjugated organic compound.. However, we need to make sure about pH of system and solvent before taking sample analysis. Using this spectroscopy, we find molar absorptivity or extinction coefficient of 2.9*10^-6 mol/L concentration Liquid seldibrdge chromophore for maximum absorption at given wavelength.